Genotyping and primer details


Genotyping and primer details for 782D10 - At3g13445
Line ID 782D10
Locus At3g13445 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4381727 of line 782D10 Chr3:4381727
   
Insertion allele amplicon 8474 - 53fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53fr: 5'-GGGCTACACACTGAGTTAAGGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4382160
   
wt amplicon (over InsPos) 26go - 53fr
wt amplicon start Chr3:4381369
wt amplicon end Chr3:4382160
wt amplicon size 792 bp
Locus specific primer 53fr: 5'-GGGCTACACACTGAGTTAAGGTTC-3'
2nd locus specific primer 26go: 5'-TGCTTTCTCAAGTGTGAGTGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11