Genotyping and primer details


Genotyping and primer details for 783C02 - At3g49810
Line ID 783C02
Locus At3g49810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18475082 of line 783C02 Chr3:18475082
   
Insertion allele amplicon 8474 - HM14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM14: 5'-CTTGTTGGGTTAATGAGATTGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18475460
   
wt amplicon (over InsPos) 04ua - HM14
wt amplicon start Chr3:18474550
wt amplicon end Chr3:18475460
wt amplicon size 911 bp
Locus specific primer HM14: 5'-CTTGTTGGGTTAATGAGATTGGTT-3'
2nd locus specific primer 04ua: 5'-AACCTCTCACAAGAACCCAAAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11