Genotyping and primer details


Genotyping and primer details for 786B08 - At5g57170
Line ID 786B08
Locus At5g57170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23162337 of line 786B08 Chr5:23162337
   
Insertion allele amplicon 8474 - 95rq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 95rq: 5'-CAAAGTGGATTTATGGGCTACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23161959
   
wt amplicon (over InsPos) 10uo - 95rq
wt amplicon start Chr5:23161959
wt amplicon end Chr5:23162695
wt amplicon size 737 bp
Locus specific primer 95rq: 5'-CAAAGTGGATTTATGGGCTACAAT-3'
2nd locus specific primer 10uo: 5'-GGCTACGTCTGTTTTCTTCTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11