Genotyping and primer details


Genotyping and primer details for 786C10 - At5g08030
Line ID 786C10
Locus At5g08030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2575443 of line 786C10 Chr5:2575443
   
Insertion allele amplicon 8474 - 32ai
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32ai: 5'-TGTTCCAACAATCAATTCAGAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2574998
   
wt amplicon (over InsPos) 21dk - 32ai
wt amplicon start Chr5:2574998
wt amplicon end Chr5:2575796
wt amplicon size 799 bp
Locus specific primer 32ai: 5'-TGTTCCAACAATCAATTCAGAGAG-3'
2nd locus specific primer 21dk: 5'-GTCTGAAGATTGGTTAAAACAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11