Genotyping and primer details


Genotyping and primer details for 787A06 - At3g55880
Line ID 787A06
Locus At3g55880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20737878 of line 787A06 Chr3:20737878
   
Insertion allele amplicon 8474 - 32ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32ve: 5'-GAGACGAAAGCTCCTAGTCTTCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20737545
   
wt amplicon (over InsPos) 38vx - 32ve
wt amplicon start Chr3:20737545
wt amplicon end Chr3:20738435
wt amplicon size 891 bp
Locus specific primer 32ve: 5'-GAGACGAAAGCTCCTAGTCTTCAA-3'
2nd locus specific primer 38vx: 5'-AAAGGAGACCAAAACCCAGTAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11