Genotyping and primer details


Genotyping and primer details for 791C05 - At5g22070
Line ID 791C05
Locus At5g22070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7308468 of line 791C05 Chr5:7308468
   
Insertion allele amplicon 8474 - 34ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34ve: 5'-AACACTCATCAGAACGATAACACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7309080
   
Insertion allele amplicon 8474 - 76vs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 76vs: 5'-CAGGTATTATAACGCTTGTCGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7307745
   
wt amplicon (over InsPos) 76vs - 34ve
wt amplicon start Chr5:7307745
wt amplicon end Chr5:7309080
wt amplicon size 1336 bp
Locus specific primer 34ve: 5'-AACACTCATCAGAACGATAACACG-3'
2nd locus specific primer 76vs: 5'-CAGGTATTATAACGCTTGTCGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11