Genotyping and primer details


Genotyping and primer details for 793E04 - At4g20720
Line ID 793E04
Locus At4g20720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11105627 of line 793E04 Chr4:11105627
   
Insertion allele amplicon 8474 - 10vb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10vb: 5'-AGCATCTTTCTCCTCAAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11106350
   
Insertion allele amplicon 8474 - 53vu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 53vu: 5'-TGTTCTTTCCCTTTGATTATTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11104927
   
wt amplicon (over InsPos) 14vj - 10vb
wt amplicon start Chr4:11105314
wt amplicon end Chr4:11106350
wt amplicon size 1037 bp
Locus specific primer 10vb: 5'-AGCATCTTTCTCCTCAAAGAA-3'
2nd locus specific primer 14vj: 5'-AGGTCTCTCCAAATTGATCATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11