Genotyping and primer details


Genotyping and primer details for 795B10 - At4g01180
Line ID 795B10
Locus At4g01180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:501698 of line 795B10 Chr4:501698
   
Insertion allele amplicon 8474 - 47uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47uj: 5'-GGAAACAATTTCTCAGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:501141
   
wt amplicon (over InsPos) 32vc - 47uj
wt amplicon start Chr4:501141
wt amplicon end Chr4:502075
wt amplicon size 935 bp
Locus specific primer 47uj: 5'-GGAAACAATTTCTCAGCTC-3'
2nd locus specific primer 32vc: 5'-GTGGGTTGTGATGGAGATAAAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11