Genotyping and primer details


Genotyping and primer details for 796D03 - At2g02730
Line ID 796D03
Locus At2g02730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:765596 of line 796D03 Chr2:765596
   
Insertion allele amplicon 8474 - NF31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NF31: 5'-GTCCCTGTTCATGAAAGAAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:765213
   
wt amplicon (over InsPos) 02dt - NF31
wt amplicon start Chr2:765213
wt amplicon end Chr2:766066
wt amplicon size 854 bp
Locus specific primer NF31: 5'-GTCCCTGTTCATGAAAGAAAGAAG-3'
2nd locus specific primer 02dt: 5'-GAGCAAAAACATGTCTGACTACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11