Genotyping and primer details


Genotyping and primer details for 798E08 - At5g04850
Line ID 798E08
Locus At5g04850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1408720 of line 798E08 Chr5:1408720
   
Insertion allele amplicon 8474 - 24dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24dr: 5'-AAAATAGCACATTGAACCAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1409139
   
wt amplicon (over InsPos) 78dy - 24dr
wt amplicon start Chr5:1408313
wt amplicon end Chr5:1409139
wt amplicon size 827 bp
Locus specific primer 24dr: 5'-AAAATAGCACATTGAACCAAAACC-3'
2nd locus specific primer 78dy: 5'-CACCATCATAAAAATCTCTCAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11