Genotyping and primer details


Genotyping and primer details for 799D01 - At3g22790
Line ID 799D01
Locus At3g22790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8052607 of line 799D01 Chr3:8052607
   
Insertion allele amplicon 8474 - 45bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45bk: 5'-TTGAATCACAGTCTGAGAAAATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8053011
   
wt amplicon (over InsPos) 06cr - 45bk
wt amplicon start Chr3:8052007
wt amplicon end Chr3:8053011
wt amplicon size 1005 bp
Locus specific primer 45bk: 5'-TTGAATCACAGTCTGAGAAAATGG-3'
2nd locus specific primer 06cr: 5'-GCTGATACTACGGTCTATTCCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11