Genotyping and primer details


Genotyping and primer details for 806D01 - At5g64220
Line ID 806D01
Locus At5g64220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25689621 of line 806D01 Chr5:25689621
   
Insertion allele amplicon 8474 - 05nj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05nj: 5'-TGTATTGGCACCATAAATGTCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25690089
   
wt amplicon (over InsPos) 11oj - 05nj
wt amplicon start Chr5:25689192
wt amplicon end Chr5:25690089
wt amplicon size 898 bp
Locus specific primer 05nj: 5'-TGTATTGGCACCATAAATGTCAGT-3'
2nd locus specific primer 11oj: 5'-AGTAGTTTCCAAGCTCCTTTCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11