Genotyping and primer details


Genotyping and primer details for 810C10 - At1g72140
Line ID 810C10
Locus At1g72140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27142733 of line 810C10 Chr1:27142733
   
Insertion allele amplicon 8474 - 07au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07au: 5'-TCTTAGGCTACACATCCATTTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27142398
   
Insertion allele amplicon 8474 - DA30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DA30: 5'-CAAAAGCATGCATGTAAATTATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27143521
   
wt amplicon (over InsPos) 07au - DA30
wt amplicon start Chr1:27142398
wt amplicon end Chr1:27143521
wt amplicon size 1124 bp
Locus specific primer DA30: 5'-CAAAAGCATGCATGTAAATTATCG-3'
2nd locus specific primer 07au: 5'-TCTTAGGCTACACATCCATTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11