Genotyping and primer details


Genotyping and primer details for 811C03 - At1g20080
Line ID 811C03
Locus At1g20080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6963740 of line 811C03 Chr1:6963740
   
Insertion allele amplicon 8474 - KU89
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU89: 5'-TGGGTTAAAAGCTACTGTTCAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6963183
   
wt amplicon (over InsPos) 07bp - KU89
wt amplicon start Chr1:6963183
wt amplicon end Chr1:6964070
wt amplicon size 888 bp
Locus specific primer KU89: 5'-TGGGTTAAAAGCTACTGTTCAGGT-3'
2nd locus specific primer 07bp: 5'-CCAATTCACAAACTGGTTTTACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11