Genotyping and primer details


Genotyping and primer details for 812F02 - At4g29450
Line ID 812F02
Locus At4g29450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14482866 of line 812F02 Chr4:14482866
   
Insertion allele amplicon 3144 - 65wl
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 65wl: 5'-GCCCTTATTCGATAGTGATACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14483414
   
Insertion allele amplicon 3144 - BB84
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BB84: 5'-ATTCTGTCCCAGATCAAAATCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14482970
   
Insertion allele amplicon 8474 - EU75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU75: 5'-CGACCAGAAGTTCACGTTTACATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14481866
   
wt amplicon (over InsPos) BB84 - EU75
wt amplicon start Chr4:14481866
wt amplicon end Chr4:14482970
wt amplicon size 1105 bp
Locus specific primer EU75: 5'-CGACCAGAAGTTCACGTTTACATA-3'
2nd locus specific primer BB84: 5'-ATTCTGTCCCAGATCAAAATCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11