Genotyping and primer details


Genotyping and primer details for 817B01 - At2g26660
Line ID 817B01
Locus At2g26660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11339743 of line 817B01 Chr2:11339743
   
Insertion allele amplicon 8474 - KU60
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU60: 5'-GGCCAGTTAAACAAATTACAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11340150
   
wt amplicon (over InsPos) 18cu - KU60
wt amplicon start Chr2:11339289
wt amplicon end Chr2:11340150
wt amplicon size 862 bp
Locus specific primer KU60: 5'-GGCCAGTTAAACAAATTACAGCAT-3'
2nd locus specific primer 18cu: 5'-TGATGATTTGGGATCAATTTGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11