Genotyping and primer details


Genotyping and primer details for 822F12 - At1g79030
Line ID 822F12
Locus At1g79030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29732698 of line 822F12 Chr1:29732698
   
Insertion allele amplicon 8474 - 42op
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42op: 5'-ATTAACAACAGTAGAAGGCGCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29732363
   
wt amplicon (over InsPos) 11vu - 42op
wt amplicon start Chr1:29732363
wt amplicon end Chr1:29733325
wt amplicon size 963 bp
Locus specific primer 42op: 5'-ATTAACAACAGTAGAAGGCGCAGT-3'
2nd locus specific primer 11vu: 5'-TTTGCTTCTGATAATGTGGAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11