Genotyping and primer details


Genotyping and primer details for 823H04 - At5g41910
Line ID 823H04
Locus At5g41910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16778519 of line 823H04 Chr5:16778519
   
Insertion allele amplicon 8474 - 07ty
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07ty: 5'-GTCTCTTCTTTCACACCTGCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16778867
   
wt amplicon (over InsPos) 06uu - 07ty
wt amplicon start Chr5:16778008
wt amplicon end Chr5:16778867
wt amplicon size 860 bp
Locus specific primer 07ty: 5'-GTCTCTTCTTTCACACCTGCTTCT-3'
2nd locus specific primer 06uu: 5'-TGACCTCAGAAAGCTAAGGAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11