Genotyping and primer details


Genotyping and primer details for 824F01 - At1g13910
Line ID 824F01
Locus At1g13910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4756542 of line 824F01 Chr1:4756542
   
Insertion allele amplicon 8474 - 62by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 62by: 5'-TGAATGTCAAAGATTACAAGTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4757008
   
wt amplicon (over InsPos) 23da - 62by
wt amplicon start Chr1:4756014
wt amplicon end Chr1:4757008
wt amplicon size 995 bp
Locus specific primer 62by: 5'-TGAATGTCAAAGATTACAAGTGGC-3'
2nd locus specific primer 23da: 5'-GTTCAATGTCTCCTTCGCTAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11