Genotyping and primer details


Genotyping and primer details for 826H06 - At5g14270
Line ID 826H06
Locus At5g14270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4606299 of line 826H06 Chr5:4606299
   
Insertion allele amplicon 3144 - 28tf
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 28tf: 5'-GCCCATAAGGAAAAACATATCGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4605938
   
Insertion allele amplicon 8474 - 35sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 35sf: 5'-CCAGGGGATAGTCTAATCACCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4606888
   
wt amplicon (over InsPos) 28tf - 35sf
wt amplicon start Chr5:4605938
wt amplicon end Chr5:4606888
wt amplicon size 951 bp
Locus specific primer 35sf: 5'-CCAGGGGATAGTCTAATCACCATA-3'
2nd locus specific primer 28tf: 5'-GCCCATAAGGAAAAACATATCGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11