Genotyping and primer details


Genotyping and primer details for 830B04 - At3g60110
Line ID 830B04
Locus At3g60110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22200175 of line 830B04 Chr3:22200175
   
Insertion allele amplicon 8474 - 38uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38uj: 5'-AAAGAATTTCCAAGGAAGACCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22199594
   
wt amplicon (over InsPos) 68wa - 38uj
wt amplicon start Chr3:22199594
wt amplicon end Chr3:22200721
wt amplicon size 1128 bp
Locus specific primer 38uj: 5'-AAAGAATTTCCAAGGAAGACCAAT-3'
2nd locus specific primer 68wa: 5'-AATATTATTCTTGGCTCTTCCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11