Genotyping and primer details


Genotyping and primer details for 832H12 - At3g51110
Line ID 832H12
Locus At3g51110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18984126 of line 832H12 Chr3:18984126
   
Insertion allele amplicon 8474 - DA42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DA42: 5'-GAAGATGATGATGATTCCAAGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18983707
   
wt amplicon (over InsPos) 09bg - DA42
wt amplicon start Chr3:18983707
wt amplicon end Chr3:18984450
wt amplicon size 744 bp
Locus specific primer DA42: 5'-GAAGATGATGATGATTCCAAGGAT-3'
2nd locus specific primer 09bg: 5'-AAGATCATCTCAGCTTCTTCTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11