Genotyping and primer details


Genotyping and primer details for 833F02 - At3g52310
Line ID 833F02
Locus At3g52310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19400021 of line 833F02 Chr3:19400021
   
Insertion allele amplicon 8474 - BF03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BF03: 5'-GCCTCTAGTGCTTCTCTTGGACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19399642
   
Insertion allele amplicon 8474 - FN40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FN40: 5'-TCTGATGAAGATGTCATTCCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19400530
   
wt amplicon (over InsPos) 02tc - BF03
wt amplicon start Chr3:19399642
wt amplicon end Chr3:19400547
wt amplicon size 906 bp
Locus specific primer BF03: 5'-GCCTCTAGTGCTTCTCTTGGACTA-3'
2nd locus specific primer 02tc: 5'-CATTCAAGATGCTCTTTTCTGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11