Genotyping and primer details


Genotyping and primer details for 834D04 - At5g22910
Line ID 834D04
Locus At5g22910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7662026 of line 834D04 Chr5:7662026
   
Insertion allele amplicon 8474 - 04iv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04iv: 5'-GCAAATGTCTTGATCCATTAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7661575
   
wt amplicon (over InsPos) 05kj - 04iv
wt amplicon start Chr5:7661575
wt amplicon end Chr5:7662344
wt amplicon size 770 bp
Locus specific primer 04iv: 5'-GCAAATGTCTTGATCCATTAGGTT-3'
2nd locus specific primer 05kj: 5'-GTATTCGTTCGATGACATTTCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11