Genotyping and primer details


Genotyping and primer details for 834F08 - At2g19410
Line ID 834F08
Locus At2g19410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8407648 of line 834F08 Chr2:8407648
   
Insertion allele amplicon 8474 - 46sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46sj: 5'-TTCGGTCTAGGAGAACAAAAGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8407963
   
wt amplicon (over InsPos) 03tf - 46sj
wt amplicon start Chr2:8407030
wt amplicon end Chr2:8407963
wt amplicon size 934 bp
Locus specific primer 46sj: 5'-TTCGGTCTAGGAGAACAAAAGGTA-3'
2nd locus specific primer 03tf: 5'-CCTAAAAATCTCTGGAGTTGTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11