Genotyping and primer details


Genotyping and primer details for 835A09 - At1g53830
Line ID 835A09
Locus At1g53830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20100226 of line 835A09 Chr1:20100226
   
Insertion allele amplicon 3144 - 08au
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 08au: 5'-CATTGACTTGGTTCAACTTGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20100850
   
Insertion allele amplicon 8474 - JF32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JF32: 5'-ACTGGAAGTAGAAATGTCGTCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20099782
   
wt amplicon (over InsPos) 08au - JF32
wt amplicon start Chr1:20099782
wt amplicon end Chr1:20100850
wt amplicon size 1069 bp
Locus specific primer JF32: 5'-ACTGGAAGTAGAAATGTCGTCGAT-3'
2nd locus specific primer 08au: 5'-CATTGACTTGGTTCAACTTGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11