Genotyping and primer details


Genotyping and primer details for 836H05 - At1g04630
Line ID 836H05
Locus At1g04630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1290586 of line 836H05 Chr1:1290586
   
Insertion allele amplicon 8474 - DA43
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DA43: 5'-TTCTGCTTGAAGAATGGGAAGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1290163
   
wt amplicon (over InsPos) 61rd - DA43
wt amplicon start Chr1:1290163
wt amplicon end Chr1:1291120
wt amplicon size 958 bp
Locus specific primer DA43: 5'-TTCTGCTTGAAGAATGGGAAGTAT-3'
2nd locus specific primer 61rd: 5'-TGGTCCTAGCCTAGTTAATCTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11