Genotyping and primer details


Genotyping and primer details for 838E03 - At5g17260
Line ID 838E03
Locus At5g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5676600 of line 838E03 Chr5:5676600
   
Insertion allele amplicon 8474 - 56kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 56kh: 5'-TGATTCCTAGCAAAGACCAAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5676975
   
wt amplicon (over InsPos) 10lb - 56kh
wt amplicon start Chr5:5676180
wt amplicon end Chr5:5676975
wt amplicon size 796 bp
Locus specific primer 56kh: 5'-TGATTCCTAGCAAAGACCAAGAAT-3'
2nd locus specific primer 10lb: 5'-CACATTGCACTTCTGTATTCACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11