Genotyping and primer details


Genotyping and primer details for 843F10 - At2g27860
Line ID 843F10
Locus At2g27860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11866149 of line 843F10 Chr2:11866149
   
Insertion allele amplicon 8474 - 46uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 46uv: 5'-GAGACTTGAAGGTCTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11866601
   
wt amplicon (over InsPos) 34vx - 46uv
wt amplicon start Chr2:11865587
wt amplicon end Chr2:11866601
wt amplicon size 1015 bp
Locus specific primer 46uv: 5'-GAGACTTGAAGGTCTTGT-3'
2nd locus specific primer 34vx: 5'-CAGTTAAAAGGTCGTACGATGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11