Genotyping and primer details


Genotyping and primer details for 844D05 - At3g03870
Line ID 844D05
Locus At3g03870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:995857 of line 844D05 Chr3:995857
   
Insertion allele amplicon 8474 - 37fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37fm: 5'-AACACCATCTCGGAAATAAAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:996413
   
wt amplicon (over InsPos) 05gf - 37fm
wt amplicon start Chr3:995382
wt amplicon end Chr3:996413
wt amplicon size 1032 bp
Locus specific primer 37fm: 5'-AACACCATCTCGGAAATAAAACAC-3'
2nd locus specific primer 05gf: 5'-TTACTCCATGAGAATACCAAACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11