Genotyping and primer details


Genotyping and primer details for 844E05 - At5g42430
Line ID 844E05
Locus At5g42430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16971540 of line 844E05 Chr5:16971540
   
Insertion allele amplicon 8474 - DA47
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DA47: 5'-CCATTTCAAGAGTTCACGGTTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4209542
   
wt amplicon (over InsPos) 63rd - DA47
wt amplicon start Chr1:4209542
wt amplicon end Chr5:16972143
wt amplicon size 1135 bp
Locus specific primer DA47: 5'-CCATTTCAAGAGTTCACGGTTAAT-3'
2nd locus specific primer 63rd: 5'-AAAGGCAGATGTCATATGTAACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11