Genotyping and primer details


Genotyping and primer details for 846H12 - At2g46140
Line ID 846H12
Locus At2g46140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18959492 of line 846H12 Chr2:18959492
   
Insertion allele amplicon 8474 - 36sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36sf: 5'-CCTCAAAATACAAAAGCTGAGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18959993
   
wt amplicon (over InsPos) 20tc - 36sf
wt amplicon start Chr2:18959018
wt amplicon end Chr2:18959993
wt amplicon size 976 bp
Locus specific primer 36sf: 5'-CCTCAAAATACAAAAGCTGAGACC-3'
2nd locus specific primer 20tc: 5'-CTCCGGCAATTAAGGTATTTATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11