Genotyping and primer details


Genotyping and primer details for 852B07 - At1g17070
Line ID 852B07
Locus At1g17070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5839322 of line 852B07 Chr1:5839322
   
Insertion allele amplicon 8474 - 48bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 48bk: 5'-GAAGAACCTCTCCATCATATCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5839689
   
wt amplicon (over InsPos) 79dy - 48bk
wt amplicon start Chr1:5838722
wt amplicon end Chr1:5839689
wt amplicon size 968 bp
Locus specific primer 48bk: 5'-GAAGAACCTCTCCATCATATCAGC-3'
2nd locus specific primer 79dy: 5'-TTGAGCATGAAATTCAAAAGATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11