Genotyping and primer details


Genotyping and primer details for 854B04 - At1g51880
Line ID 854B04
Locus At1g51880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19273272 of line 854B04 Chr1:19273272
   
Insertion allele amplicon 3144 - BB86
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BB86: 5'-CAACTTCAACCTTACCGCTAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19273693
   
Insertion allele amplicon 8474 - EU82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU82: 5'-TCATAGGGGTTACTTGTATCGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19272924
   
wt amplicon (over InsPos) BB86 - EU82
wt amplicon start Chr1:19272924
wt amplicon end Chr1:19273693
wt amplicon size 770 bp
Locus specific primer EU82: 5'-TCATAGGGGTTACTTGTATCGACC-3'
2nd locus specific primer BB86: 5'-CAACTTCAACCTTACCGCTAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11