Genotyping and primer details


Genotyping and primer details for 854B10 - At5g50330
Line ID 854B10
Locus At5g50330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20488365 of line 854B10 Chr5:20488365
   
Insertion allele amplicon 8474 - 40uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40uj: 5'-AATGTTGCTACAGTTCCATGTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20487765
   
wt amplicon (over InsPos) 40vc - 40uj
wt amplicon start Chr5:20487765
wt amplicon end Chr5:20488745
wt amplicon size 981 bp
Locus specific primer 40uj: 5'-AATGTTGCTACAGTTCCATGTCAG-3'
2nd locus specific primer 40vc: 5'-GGATTGCTAAGCTTGTCTGAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11