Genotyping and primer details


Genotyping and primer details for 855B01 - At1g10490
Line ID 855B01
Locus At1g10490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3459722 of line 855B01 Chr1:3459722
   
Insertion allele amplicon 8474 - 66gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 66gp: 5'-AACCAGTTTCACACATTGCAGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3460160
   
wt amplicon (over InsPos) 12hs - 66gp
wt amplicon start Chr1:3459264
wt amplicon end Chr1:3460160
wt amplicon size 897 bp
Locus specific primer 66gp: 5'-AACCAGTTTCACACATTGCAGTAG-3'
2nd locus specific primer 12hs: 5'-GAGGGGCCAAATATACTCTCTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11