Genotyping and primer details


Genotyping and primer details for 856G10 - At3g01770
Line ID 856G10
Locus At3g01770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:276002 of line 856G10 Chr3:276002
   
Insertion allele amplicon 8474 - 65sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 65sm: 5'-AATCGATTTGCGGATATAATCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:276353
   
wt amplicon (over InsPos) 13ti - 65sm
wt amplicon start Chr3:275567
wt amplicon end Chr3:276353
wt amplicon size 787 bp
Locus specific primer 65sm: 5'-AATCGATTTGCGGATATAATCTTG-3'
2nd locus specific primer 13ti: 5'-CAACACTCATGAATCTCAGTCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11