Genotyping and primer details


Genotyping and primer details for 857F12 - At5g25030
Line ID 857F12
Locus At5g25030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8620678 of line 857F12 Chr5:8620678
   
Insertion allele amplicon 8474 - 39sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39sf: 5'-ACTAATGCATCCCTGTGATGTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8621073
   
wt amplicon (over InsPos) 15tc - 39sf
wt amplicon start Chr5:8620141
wt amplicon end Chr5:8621073
wt amplicon size 933 bp
Locus specific primer 39sf: 5'-ACTAATGCATCCCTGTGATGTGTA-3'
2nd locus specific primer 15tc: 5'-CTTCCACTTTCTCTTTTGTTGGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11