Genotyping and primer details


Genotyping and primer details for 860A04 - At1g58684
Line ID 860A04
Locus At1g58684 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21770190 of line 860A04 Chr1:21770190
   
Insertion allele amplicon 8474 - 08yj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08yj: 5'-CGGTGACCGTGGTGACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21689153
   
Insertion allele amplicon 8474 - 12yk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12yk: 5'-TGCACTCAATTTGCATAAAGTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21771504
   
Insertion allele amplicon 8474 - 83yh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 83yh: 5'-TGGTGATAAATGAAGCGTCTAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21770397
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11