Genotyping and primer details


Genotyping and primer details for 861E01 - At4g22070
Line ID 861E01
Locus At4g22070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11693741 of line 861E01 Chr4:11693741
   
Insertion allele amplicon 8474 - IS83
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS83: 5'-TTGATGAGGTTGACTTCTTCTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11694146
   
wt amplicon (over InsPos) 23rd - IS83
wt amplicon start Chr4:11693427
wt amplicon end Chr4:11694146
wt amplicon size 720 bp
Locus specific primer IS83: 5'-TTGATGAGGTTGACTTCTTCTCAG-3'
2nd locus specific primer 23rd: 5'-TGTGCGTTCTTCATTTCATTTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11