Genotyping and primer details


Genotyping and primer details for 863E04 - At5g63140
Line ID 863E04
Locus At5g63140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25329549 of line 863E04 Chr5:25329549
   
Insertion allele amplicon 8474 - 26ey
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26ey: 5'-CATGACATTGTTGTTTTAACCCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25329104
   
wt amplicon (over InsPos) 02gc - 26ey
wt amplicon start Chr5:25329104
wt amplicon end Chr5:25329873
wt amplicon size 770 bp
Locus specific primer 26ey: 5'-CATGACATTGTTGTTTTAACCCTG-3'
2nd locus specific primer 02gc: 5'-TGGTAACACAAAATGGTAAAACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11