Genotyping and primer details


Genotyping and primer details for 864E08 - At1g10640
Line ID 864E08
Locus At1g10640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3516293 of line 864E08 Chr1:3516293
   
Insertion allele amplicon 8474 - 23qs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23qs: 5'-CAGGTGAGAATTGATCTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3516850
   
wt amplicon (over InsPos) 50ve - 23qs
wt amplicon start Chr1:3515770
wt amplicon end Chr1:3516850
wt amplicon size 1081 bp
Locus specific primer 23qs: 5'-CAGGTGAGAATTGATCTCTTT-3'
2nd locus specific primer 50ve: 5'-TTGCTATGGTCACAGTAGAATTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11