Genotyping and primer details


Genotyping and primer details for 865E01 - At2g19390
Line ID 865E01
Locus At2g19390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8395572 of line 865E01 Chr2:8395572
   
Insertion allele amplicon 8474 - 26dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26dr: 5'-GTATTGCTCTTGGAATACTAGGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8396209
   
wt amplicon (over InsPos) 04es - 26dr
wt amplicon start Chr2:8395010
wt amplicon end Chr2:8396209
wt amplicon size 1200 bp
Locus specific primer 26dr: 5'-GTATTGCTCTTGGAATACTAGGCG-3'
2nd locus specific primer 04es: 5'-CTCGGTAACCATCAACCACTTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11