Genotyping and primer details


Genotyping and primer details for 867H11 - At3g12977
Line ID 867H11
Locus At3g12977 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4144906 of line 867H11 Chr3:4144906
   
Insertion allele amplicon 8474 - 07ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07ux: 5'-AAATATGTTGGAGAAAATACCCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4144535
   
wt amplicon (over InsPos) 53vy - 07ux
wt amplicon start Chr3:4144535
wt amplicon end Chr3:4145602
wt amplicon size 1068 bp
Locus specific primer 07ux: 5'-AAATATGTTGGAGAAAATACCCCC-3'
2nd locus specific primer 53vy: 5'-GATGTAAGAGTCCATGAATGCAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11