Genotyping and primer details


Genotyping and primer details for 870B10 - At5g48970
Line ID 870B10
Locus At5g48970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19858790 of line 870B10 Chr5:19858790
   
Insertion allele amplicon 8474 - CN72
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN72: 5'-CTTTGGATTGTATTGTGGAGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19859146
   
wt amplicon (over InsPos) 04tx - CN72
wt amplicon start Chr5:19858302
wt amplicon end Chr5:19859146
wt amplicon size 845 bp
Locus specific primer CN72: 5'-CTTTGGATTGTATTGTGGAGGTTT-3'
2nd locus specific primer 04tx: 5'-TTACAGGTTCATAAGTCACGCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11