Genotyping and primer details


Genotyping and primer details for 870C11 - At2g23350
Line ID 870C11
Locus At2g23350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9945120 of line 870C11 Chr2:9945120
   
Insertion allele amplicon 8474 - 49bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49bk: 5'-AGTGGTACTAGCAAAGGATCAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9944691
   
wt amplicon (over InsPos) 31da - 49bk
wt amplicon start Chr2:9944691
wt amplicon end Chr2:9945729
wt amplicon size 1039 bp
Locus specific primer 49bk: 5'-AGTGGTACTAGCAAAGGATCAGGA-3'
2nd locus specific primer 31da: 5'-TCCTACATAGCAGCAAGTCAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11