Genotyping and primer details


Genotyping and primer details for 876H04 - At5g51560
Line ID 876H04
Locus At5g51560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20946577 of line 876H04 Chr5:20946577
   
Insertion allele amplicon 8474 - CN31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN31: 5'-GGGAAAGGCTTATCTGGTAAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20946044
   
Insertion allele amplicon 8474 - EU89
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU89: 5'-TGAGAAACACTTCACAAAATACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20947147
   
wt amplicon (over InsPos) CN31 - EU89
wt amplicon start Chr5:20946044
wt amplicon end Chr5:20947147
wt amplicon size 1104 bp
Locus specific primer EU89: 5'-TGAGAAACACTTCACAAAATACGC-3'
2nd locus specific primer CN31: 5'-GGGAAAGGCTTATCTGGTAAAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11