Genotyping and primer details


Genotyping and primer details for 877A06 - At5g26910
Line ID 877A06
Locus At5g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9466607 of line 877A06 Chr5:9466607
   
Insertion allele amplicon 8474 - 32fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32fr: 5'-AAGTTAGCAGCATTAGCACTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9467179
   
wt amplicon (over InsPos) 06gl - 32fr
wt amplicon start Chr5:9466301
wt amplicon end Chr5:9467179
wt amplicon size 879 bp
Locus specific primer 32fr: 5'-AAGTTAGCAGCATTAGCACTGTGA-3'
2nd locus specific primer 06gl: 5'-CTCAATGTCAATGCCTTCTTCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11