Genotyping and primer details


Genotyping and primer details for 881A04 - At3g51080
Line ID 881A04
Locus At3g51080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18974204 of line 881A04 Chr3:18974204
   
Insertion allele amplicon 8474 - 41uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41uj: 5'-AGAAAACCTAATTTGTGTTGGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18973599
   
wt amplicon (over InsPos) 39vc - 41uj
wt amplicon start Chr3:18973599
wt amplicon end Chr3:18974604
wt amplicon size 1006 bp
Locus specific primer 41uj: 5'-AGAAAACCTAATTTGTGTTGGTGG-3'
2nd locus specific primer 39vc: 5'-TCAGAAGTCTCTTTCTTCCTACGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11