Genotyping and primer details


Genotyping and primer details for 881C01 - At4g34265
Line ID 881C01
Locus At4g34265 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16402301 of line 881C01 Chr4:16402301
   
Insertion allele amplicon 8474 - 16ed
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16ed: 5'-CAAAACTTTAAAAACTTGGCCACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16402836
   
wt amplicon (over InsPos) 01ej - 16ed
wt amplicon start Chr4:16402028
wt amplicon end Chr4:16402836
wt amplicon size 809 bp
Locus specific primer 16ed: 5'-CAAAACTTTAAAAACTTGGCCACT-3'
2nd locus specific primer 01ej: 5'-GGAACCCGAGAAAGTATCTCACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11